Fluorescence Spectrometric Determination of Drugs Containing 𝜶-Methylene Sulfone/Sulfonamide Functional Groups Using N¹-Methylnicotinamide Chloride as a Fluorogenic Agent

Table 1

Optimum conditions for the fluorometric procedure.

Drug	pH*	NaOH conc. (M)	NaOH volume (mL)	NMNCl conc. (mM)	NMNCl volume (mL)	Cooling time (min)	Heating time (min)	𝜆ex (nm)	𝜆em (nm)
1	3.0	6.0	1.1	1.0	0.9	9	6	350	395
2	3.2	5.0	1.0	2 × 10^-1	1.0	10	3	336	391
3	2.5	7.0	0.9	4 × 10^-1	1.2	8	5	354	440
4	1.5	8.0	1.0	5 × 10^-1	0.9	7	3	325	375

*The reaction pH.