Fluorescence Spectrometric Determination of Drugs Containing 𝜶-Methylene Sulfone/Sulfonamide Functional Groups Using N¹-Methylnicotinamide Chloride as a Fluorogenic Agent

Table 4

Recovery data of 1–4 in spiked human plasma samples using the proposed method.

Drug	Claimed drug concentration	*Recovered concentration	% Recovery	Mean % recovery ± S.D.	C.V.
1 (μg/mL)	5	5.02	100.4%	𝟏𝟎𝟎.𝟑±𝟐.𝟎	2.0%
	20	20.20	101.0%
	60	59.00	98.3%
	80	78.00	97.5%
	100	102.30	102.3%
	150	153.00	102.0%
2 (ng/mL)	10	9.8	98.00%	𝟗𝟗.𝟗𝟖 ±𝟏.𝟗	1.9%
	30	30.5	101.60%
	50	51.0	102.00%
	100	102.0	102.00%
	300	295.0	98.30%
	500	490.0	98.00%
	800	810.0	101.20%
	1000	987.0	98.70%
3 (ng/mL)	10	10.2	102.0%	𝟏𝟎𝟎.𝟒 ±𝟏.𝟒	1.4%
	100	102.0	102.0%
	300	297.0	99.0%
	800	807.0	100.9%
	1000	991.0	99.1%
	1500	1515.0	101.0%
	1700	1685.0	99.1%
4 (ng/mL)	30	29.6	98.7%	𝟗𝟗.𝟗𝟔±𝟏.𝟓	1.5%
	100	98.0	98.0%
	500	509.0	101.8%
	1000	1015.0	101.0%
	1200	1181.0	98.4%
	1500	152.5	101.6%
	1800	1809.0	100.5%
	2100	2345.0	99.7%

*Average of triplicate analyses.