Fluorescence Spectrometric Determination of Drugs Containing 𝜶-Methylene Sulfone/Sulfonamide Functional Groups Using N¹-Methylnicotinamide Chloride as a Fluorogenic Agent

Table 5

Intraday and interday precision of 1–4 determination in plasma samples using the proposed method.

Drug	Claimed conc.		Intraday			Interday
		Found conc*	S.D.	C.V.	Found conc*	S.D.	C.V.
1 (μg/mL)	5	5.03	0.02	0.4%	4.9	0.71	0.14%
	20	20.1	0.07	0.4%	19.7	0.21	1.10%
	60	61.3	0.90	1.5%	58.6	0.99	1.70%
	80	78.0	0.14	1.7%	83.0	2.12	2.60%
	100	104.0	2.80	2.8%	97.0	2.12	2.15%
	150	154.0	2.80	1.9%	146.0	2.80	1.90%
2 (ng/mL)	10	9.7	0.21	2.2%	9.8	0.14	1.42%
	30	29.3	0.50	1.7%	30.5	0.35	1.16%
	50	51.5	1.10	2.1%	49.0	0.71	1.43%
	100	102.0	1.40	1.4%	102.0	1.40	1.40%
	300	296.0	2.80	1.0%	289.0	7.78	2.60%
	500	512.0	8.40	1.7%	510.0	7.10	1.40%
	800	795.0	3.50	0.4%	810.0	7.10	1.87%
	1000	985.0	10.60	1.1%	985.0	10.60	1.10%
3 (ng/mL)	10	10.4	0.30	2.8%	10.3	0.21	2.08%
	100	96.0	2.80	2.9%	96.0	2.82	2.90%
	300	304.0	2.80	0.9%	305.0	3.50	1.20%
	500	495.0	0.35	0.7%	506.0	4.24	0.84%
	1000	1020.0	14.10	1.4%	1020.0	14.10	1.40%
	1100	1125.0	17.70	1.6%	1125.0	17.70	1.60%
	1500	1475.0	17.70	1.2%	1485.0	10.60	0.71%
	1700	1720.0	14.10	0.8%	1720.0	14.10	0.83%
4 (ng/mL)	30	29.4	0.40	1.40%	30.3	0.21	0.70%
	100	98.0	1.40	1.40%	104.0	2.83	2.80%
	500	505.0	3.50	0.70%	491.0	6.36	1.28%
	1000	1015.0	10.60	1.10%	980.0	14.10	1.40%
	1200	1185.0	10.60	0.90%	1225.0	17.70	1.45%
	1500	1524.0	16.90	1.10%	1529.0	20.50	1.35%
	1800	1815.0	10.60	0.60%	1780.0	14.10	0.79%
	2350	2340.0	7.10	0.30%	2360.0	7.10	0.30%

*Average of triplicate analyses.