Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products

Table 1

Effect of acetic acid percentage in phase B of mobile phase on pH, resolution factors, and total analysis time.

Acetic acid concentration (%, v/v)	0	0.5	1	1.5	2
pH value	7	4	3.5	3.2	3
Resolution factors (𝑅𝑠)	0.79	1.92	1.98	1.99	2
Total time of elute the analytes (minutes)	8.5	6.0	5.5	5.3	5.3