Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products

Table 5

Concentration of BHA, BHT, and OMC in sunscreen cream, milk lotion, hair gel, and hair oil samples determined by RP-HPLC-UV/Vis at 𝜆_max = 280 nm.

Type	Commercial name	Country of origin	Phenolic compounds	Mean concentration (mg/g)
				(1) (𝑛=4)	(2) (𝑛=4)	(3) (𝑛=4)	Average (mg/g)	RSD%
Sunscreen cream	Aiken	Malaysia	BHA	4.80±0.10	4.90±0.07	4.90±0.05	4.85	1.50
			BHT	1.30±0.06	1.40±0.07	1.28±0.03	1.33	3.88
			OMC	62.1±0.60	65.9±0.41	68.5±0.51	65.5	0.77
	Nivea	Thailand	BHA	3.31±0.09	3.03±0.08	3.43±0.07	3.26	2.43
			BHT	1.16±0.06	1.03±0.04	0.85±0.04	1.01	4.47
			OMC	27.68±0.4	30.72±0.3	25.48±0.6	27.96	1.58
	Gervenne	Malaysia	BHA	1.93±0.08	1.81±0.06	1.72±0.08	1.82	3.92
			BHT	n.d	n.d	n.d	n.d	n.d
			OMC	16.66±0.4	14.61±0.5	17.43±0.4	16.23	2.68
Milk lotion	Nivea	Thailand	BHA	4.51±0.12	4.46±0.05	4.55±0.04	4.50	1.57
			BHT	1.96±0.09	2.58±0.07	2.37±0.06	2.30	3.21
			OMC	13.4±0.26	12.5±0.17	15.6±0.21	13.83	1.55
	New Trendy	Malaysia	BHA	3.92±0.15	4.15±0.11	4.42±0.09	4.16	2.82
			BHT	n.d	n.d	n.d	n.d	n.d
			OMC	7.82±0.38	8.68±0.32	10.48±0.31	8.99	3.79
	Garnier	Indonesia	BHA	2.96±0.09	2.47±0.10	2.79±0.09	2.74	3.32
			BHT	0.64±0.03	0.83±0.02	0.71±0.03	0.73	3.26
			OMC	20.41±0.38	16.64±0.30	15.13±0.30	17.0	1.86
Hair gel	De Boy	Malaysia	BHA	1.23±0.05	1.27±0.04	1.33±0.04	1.28	3.14
			BHT	0.17±0.01	0.24±0.01	0.26±0.01	0.22	3.40
			OMC	0.11±0.01	0.15±0.01	0.12±0.01	0.13	4.52
	Beyond	Malaysia	BHA	1.28±0.04	1.36±0.06	1.49±0.05	1.38	3.37
			BHT	0.13±0.01	0.19±0.01	0.16±0.01	0.16	4.05
			OMC	0.31±0.01	0.24±0.01	0.36±0.02	0.30	3.48
	Elite	Malaysia	BHA	1.42±0.06	1.48±0.03	1.63±0.04	1.51	2.76
			BHT	0.17±0.01	0.11±0.01	0.23±0.01	0.17	4.48
			OMC	0.81±0.03	0.93±0.02	0.79±0.02	0.84	2.69
Hair oil	Elite	Malaysia	BHA	3.96±0.04	3.93±0.03	3.85±0.05	3.89	1.06
			BHT	0.89±0.02	0.87±0.02	0.84±0.01	0.87	2.11
			OMC	0.83±0.02	0.82±0.01	0.80±0.01	0.82	1.37
	Gervenne	Malaysia	BHA	0.11±0.01	0.12±0.01	0.15±0.01	0.13	4.66
			BHT	1.44±0.05	1.61±0.05	1.57±0.06	1.54	3.25
			OMC	3.42±0.06	3.29±0.07	3.48±0.05	3.40	1.75
	Johnsons	Philippines	BHA	0.34±0.01	0.29±0.01	0.26±0.01	0.30	3.40
			BHT	0.19±0.01	0.22±0.01	0.14±0.01	0.18	4.13
			OMC	0.51±0.02	0.63±0.01	0.56±0.01	0.57	2.19

n.d: not detected or below detection limit.