Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract

Figure 4

Double Eastern blotting staining of ginsenosides contained in various ginseng samples using anti-ginseenoside-Rb1 and anti-ginsenoside-Rg₁ MAbs. (a) TLC profile stained by sulfuric acid. (b) Eastern blotting by anti-ginsenoside-Rb₁ and anti-ginseside-Rg₁ monoclonal antibodies I, II, III, IV, V, and VI indicated white ginseng, red ginseng, fibrous ginseng (P. ginseng), P. notoginseng, P. quinquefolius and P. japonicus, respectively. Upper purple color spots and lower blue color spots were stained by anti-ginsenoside-Rg₁, and anti-ginsenoside-Rb₁ monoclonal antibodies, respectively.

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(b)