Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract
Double Eastern blotting staining of ginsenosides contained in various ginseng samples using anti-ginseenoside-Rb1 and anti-ginsenoside-Rg1 MAbs. (a) TLC profile stained by sulfuric acid. (b) Eastern blotting by anti-ginsenoside-Rb1 and anti-ginseside-Rg1 monoclonal antibodies I, II, III, IV, V, and VI indicated white ginseng, red ginseng, fibrous ginseng (P. ginseng), P. notoginseng, P. quinquefolius and P. japonicus, respectively. Upper purple color spots and lower blue color spots were stained by anti-ginsenoside-Rg1, and anti-ginsenoside-Rb1 monoclonal antibodies, respectively.
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