Figure 10: Pharmacologic effects on nanosensors at 25°C. Cells incubated at 25°C with the addition of bafilomycin or amiloride. Cells are incubated at 25°C with simultaneous addition of nanosensors and respective drug. (a) Is a demonstration of total nanosensor number displayed as pixels, averaged from three individual cells. (b) Whole-cell pH image maps of cells incubated at 25°C with the addition of nanosensors alone (untreated), with bafilomycin, or amiloride, followed by imaging at the corresponding time points below. (c) Bar graph demonstration of the percentage of nanosensors averaged from three cells, from the whole, that fall into each pH grouping in cells that are either untreated and incubated at 25°C, or treated with drug and incubated at 25°C. Reprinted with permission from Nowak-Lovato et al. [11] (copyright 2010 Springer).