Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations

Figure 1

Electropherogram of standard mixture in acidic BGE. Peak identification: (1) B₁ 20 μg/mL; (2) B₃ 10 μg/mL; (3) B₆ 5 μg/mL; (4) B _12a 20 μg/mL; (5) B _1a10 μg/mL; (6) B₁₀ 5 μg/mL; (7) B₉ 15 μg/mL; (8) B₂ + B₁₂ (10 + 20) μg/mL; (9) B₇ 10 μg/mL; (10) B₅ 40 μg/mL. Experimental conditions: phosphate buffer 20 mM; pH = 2.3;𝑉 = 30 kV; 𝑖 = 80 μA; 𝑇 = 25°C; injection at 0.5 psi for 5 s, fused silica capillary i.d. = 50 μm, 𝐿 = 59.5 cm, 𝑙 = 49.5 cm instrument P/ACE MDQ System (Beckman).