Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma

Table 3

Robustness of the proposed methods.

Method	Experimental parameter variation	Recovery (%) ± SD^a
	No variation^b	101.07±0.34
	SDS volume (μL)
	90	96.10±1.13
	110	99.16±0.44
	pH
Micelle-enhanced spectrofluorometric	5.3	101.23±1.27
	5.7	98.52±0.55
	Temperature (°C)
	20	102.13±0.91
	30	100.81±1.92
	No variation^b	101.57±0.53
	pH
	3.3	100.05±1.83
	3.7	101.02±2.79
	Acetylacetone volume (mL)
	0.4	99.16±1.40
	0.6	99.86±0.91
Hantzsch reaction	Formaldehyde volume (mL)
	0.8	97.60±0.92
	1.2	100.90±2.30
	Temperature (°C)
	95	97.06±1.40
	105	102.36±1.90
	Heating time (min)
	18	99.31±2.74
	22	102.33±1.83

aMean of three determinations.
bFollowing the general assay procedure conditions.