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International Journal of Analytical Chemistry
Volume 2014 (2014), Article ID 109592, 6 pages
Research Article

Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination

1Department of Chemistry, University of Balochistan, Sariab Road, Quetta 87300, Pakistan
2Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan
3Department of Biosciences, COMSATS Institute of Information Technology, Islamabad 45320, Pakistan
4Department of Chemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan

Received 17 September 2014; Accepted 13 December 2014; Published 28 December 2014

Academic Editor: Peter A. Tanner

Copyright © 2014 Muhammad Asgher et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB) which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2) and hydrogen peroxide (H2O2). Highly reactive hydroxyl radicals (OH) were produced via dismutation of H2O2 by catalyst (Cu2+). The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, ) of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL−1, respectively. The sample throughput of 70 h−1 for vitamins A and C and 30 h−1 for RB was found. Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50 μg mL−1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; ) in the range 1.6–3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values.