Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination

Figure 1

FI-CL manifold for the determination of retinol. R1 = pyrophosphate buffer (0.01 M, pH 10.25), R2 = RB (1 × 10⁻⁴ M) containing Cu²⁺ (5 × 10⁻⁴ M), R3 = luminol solution (5 × 10⁻⁵ M), CR = chart recorder, HV = high voltage power supply, and PMT = photomultiplier tube detector.