Bioactive Paper Sensor Based on the Acetylcholinesterase for the Rapid Detection of Organophosphate and Carbamate Pesticides

Figure 2

Acetylcholinesterase (AChE) hydrolyzes the acetylthiocholine and forms thiocholine base, which then reacts with dithiobisnitrobenzoate (DTNB) to generate 5-thio-2-nitrobenzoate (TNB, an anion), which is yellow in color.