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International Journal of Analytical Chemistry
Volume 2015 (2015), Article ID 170239, 7 pages
Review Article

Spectrophotometric Analysis of Caffeine

1Department of Chemistry, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak (UNIMAS), 94300 Sarawak, Malaysia
2School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia

Received 1 September 2015; Revised 13 October 2015; Accepted 13 October 2015

Academic Editor: Josep Esteve-Romero

Copyright © 2015 Showkat Ahmad Bhawani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The nature of caffeine reveals that it is a bitter white crystalline alkaloid. It is a common ingredient in a variety of drinks (soft and energy drinks) and is also used in combination with various medicines. In order to maintain the optimum level of caffeine, various spectrophotometric methods have been developed. The monitoring of caffeine is very important aspect because of its consumption in higher doses that can lead to various physiological disorders. This paper incorporates various spectrophotometric methods used in the analysis of caffeine in various environmental samples such as pharmaceuticals, soft and energy drinks, tea, and coffee. A range of spectrophotometric methodologies including chemometric techniques and derivatization of spectra have been used to analyse the caffeine.