Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (<i>Cynodon dactylon</i> Linn.) (Pers.)

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left" rowspan="2">Proton number</td><td align="center" colspan="2">Chemical shift (<svg height="15.2665pt" id="M10" style="vertical-align:-5.5886pt" version="1.1" viewbox="-0.0498162 -9.6779 24.8419 15.2665" width="24.8419pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M494 514C482 587 419 712 303 712C238 712 174 667 174 603C174 561 205 514 249 449C219 438 187 422 162 407C93 366 23 283 23 177C23 69 87 -12 190 -12C244 -12 288 5 328 33C406 87 444 170 444 249C444 329 404 391 331 475C265 550 222 605 222 627C222 647 238 657 267 657C355 657 421 585 484 499L494 514ZM359 234C359 143 319 30 219 30C172 30 114 75 114 178C114 275 163 343 195 378C212 397 241 415 269 425C305 382 359 313 359 234Z" id="g113-226"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="517" vert-adv-y="517"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,6.666,3.264)"><path d="M169 380V459C122 440 66 423 24 416V392C86 384 90 382 90 317V-135C90 -201 81 -207 17 -213V-240H253V-213C176 -207 169 -201 169 -125V6C182 -1 208 -11 238 -12C368 12 487 109 487 260C487 358 421 449 310 449C298 449 279 444 261 433L169 380ZM169 346C196 367 237 389 269 389C341 389 403 329 403 221C403 109 347 37 263 37C228 37 191 53 169 76V346Z" id="g190-113"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="524" vert-adv-y="524"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,11.53,3.264)"><path d="M169 380V459C122 440 66 423 24 416V392C86 384 90 382 90 317V-135C90 -201 81 -207 17 -213V-240H253V-213C176 -207 169 -201 169 -125V6C182 -1 208 -11 238 -12C368 12 487 109 487 260C487 358 421 449 310 449C298 449 279 444 261 433L169 380ZM169 346C196 367 237 389 269 389C341 389 403 329 403 221C403 109 347 37 263 37C228 37 191 53 169 76V346Z" id="g190-113"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="524" vert-adv-y="524"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,16.386,3.264)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z" id="g190-110"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="819" vert-adv-y="819"></glyph.data></g></svg>)</td></tr><tr class="thead"><td align="center">Theoretical<br/>[<a href="/journals/ijac/2015/201386/#B29">29</a>, <a href="/journals/ijac/2015/201386/#B30">30</a>]</td><td align="center">Experimental (isolated compound)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">1</td><td align="center">12.13</td><td align="center">—</td></tr><tr><td align="left">2</td><td align="center">8.10</td><td align="center">7.341</td></tr><tr><td align="left">3</td><td align="center">6.97</td><td align="center">6.689</td></tr><tr><td align="left">4</td><td align="center">3.56</td><td align="center">3.219</td></tr><tr><td align="left">5</td><td align="center">7.06</td><td align="center">6.725</td></tr><tr><td align="left">6</td><td align="center">7.65</td><td align="center">7.364</td></tr><tr><td align="left">7</td><td align="center">6.44</td><td align="center">6.199</td></tr><tr><td align="left"> 8</td><td align="center">8.15</td><td align="center">7.521</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Chemical shifts of P-coumaric acid in H<sup>1</sup> NMR spectra, <svg height="9.89089pt" id="M9" style="vertical-align:-0.2129898pt" version="1.1" viewbox="-0.0498162 -9.6779 7.13029 9.89089" width="7.13029pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M494 514C482 587 419 712 303 712C238 712 174 667 174 603C174 561 205 514 249 449C219 438 187 422 162 407C93 366 23 283 23 177C23 69 87 -12 190 -12C244 -12 288 5 328 33C406 87 444 170 444 249C444 329 404 391 331 475C265 550 222 605 222 627C222 647 238 657 267 657C355 657 421 585 484 499L494 514ZM359 234C359 143 319 30 219 30C172 30 114 75 114 178C114 275 163 343 195 378C212 397 241 415 269 425C305 382 359 313 359 234Z" id="g113-226"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="517" vert-adv-y="517"></glyph.data></g></svg> (ppm).

International Journal of Analytical Chemistry

tab2

Table 2

Table 2: Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (<i>Cynodon dactylon</i> Linn.) (Pers.) 

Table 2 | Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.) 