Highly Sensitive Immunochromatographic Identification of Tetracycline Antibiotics in Milk

Table 1

Antibody immobilization characteristics on the GNP surface.

Concentration of antibodies when conjugated, g/mL	4.0	8.0
Molar ratio of IgG : GNP when conjugated	25 : 1	50 : 1
Residual concentration of antibodies determined in the supernatant, µg/mL	0.22	0.47
Antibody immobilization degree, %	94.5	94.1
Actual molar ratio of IgG : GNP in the conjugate	24 : 1	47 : 1