Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation

Table 1

RT-qPCR results using spin and vacuum filtration based protocols for HCV-RNA extraction.

Sample ID	CT; Internal control	CT	CT	IU mL^−1	IU mL^−1	Cv %
		Vacuum filtration	Spin	Vacuum filtration	Spin
1	25.99	15.6 ± 0.3	18.4 ± 0.4	481,419	67,458	0.9
2	26.48	16.0 ± 0.3	17.0 ± 0.3	360,304	177,932	0.6
3	26.57	22.6 ± 0.7	24.7 ± 0.5	3,602	847	0.01
4	26.8	12.8 ± 0.2	15.1 ± 0.3	3,299,175	704,535	6.4
5	27.5	18.6 ± 0.4	19.6 ± 0.4	60,629	28,823	0.1
6	26.34	13.3 ± 0.2	15.7 ± 0.3	2,461,014	430,986	4.7
7	27.14	16.1 ± 0.3	18.8 ± 0.4	348,791	51,043	0.7
8	26.86	17.6 ± 0.3	18.6 ± 0.4	112,812	56,348	0.2
9	27.84	14.6 ± 0.2	16.8 ± 0.3	946,830	206,548	1.8
10	26.54	17.2 ± 0.3	31.8 ± 1.2	160,482	54,132	0.3
11	26.29	19.2 ± 0.4	20.4 ± 0.6	38,131	16,544	0.1
12	24.9	21.4 ± 0.6	22.18 ± 0.7	9,683	5,736	0.0
13	24.7	15.07 ± 0.3	17.2 ± 0.3	767,124	179,381	0.2
14	26.28	18.9 ± 0.4	19.8 ± 0.4	52,693	29,976	0.02
15	26.77	22.2 ± 0.7	22.6 ± 0.7	5,810	4,340	0.0
16	26.2	20.4 ± 0.6	21.2 ± 0.6	19,312	11,584	0.01
17	25.4	19.8 ± 0.4	20.0 ± 0.4	29,849	25,136	0.01
18	26.8	21.6 ± 0.6	21.8 ± 0.7	8,328	7,507	0.00
19	26.1	15.3 ± 0.3	17.1 ± 0.3	642,090	187,782	0.2
20	26.0	21.4 ± 0.6	23.4 ± 0.7	9,533	2,555	0.0
21	26.1	18.8 ± 0.4	19.0 ± 0.4	55,731	50,456	0.02
22	26.1	17.2 ± 0.3	18.3 ± 0.4	171,609	83,106	0.06
23	25.7	19.3 ± 0.4	20.1 ± 0.4	46,845	24,354	0.02
24	28.4	21.5 ± 0.6	22.5 ± 0.7	9,201	4,760	0.0
25	26.1	22.1 ± 0.6	24.3 ± 0.7	5,980	1,307	0.0
26	26.8	20.3 ± 0.6	20.7 ± 0.6	20,985	16,260	0.01
27	31.5	26.0 ± 0.9	26.9 ± 0.8	280	150	0.01
28	18.5	18.1 ± 0.3	18.5 ± 0.4	95,495	70,695	0.04
29	31.2	16.2 ± 0.3	17.5 ± 0.3	352,282	145,151	0.1
30	27.5	19.44 ± 0.4	20.5 ± 0.6	37,850	18,256	0.01