An Optimized High Throughput Clean-Up Method Using Mixed-Mode SPE Plate for the Analysis of Free Arachidonic Acid in Plasma by LC-MS/MS

Figure 5

Chromatograms of arachidonic acid in different samples. a: unspiked plasma of Method C, b: unspiked plasma of Method B, c: unspiked sample of Method A, d: spiked plasma of Method D at a concentration of 500 ng/mL, e: unspiked plasma of Method D, and f: arachidonic acid standard, 500 ng/mL.