An Optimized High Throughput Clean-Up Method Using Mixed-Mode SPE Plate for the Analysis of Free Arachidonic Acid in Plasma by LC-MS/MS

Table 1

MS parameters.

Analyte	/min	Q1	Q3	DP	CE	IS/V	TEM/°C	GS1/Pa	GS2/Pa	CUR/Pa
Arachidonic acid	3.3	303	259.1	−109	−18	−4500	500	55	35	15
		303	205.1	−107	−20
Phospholipids	4.1	496.3	184.3	63	20	+5500	600	50	50	15