An Optimized High Throughput Clean-Up Method Using Mixed-Mode SPE Plate for the Analysis of Free Arachidonic Acid in Plasma by LC-MS/MS

Table 2

Recoveries of arachidonic acid in spiked samples treated by four sample pretreatment methods.

Concentration of arachidonic acid spiked in plasma	PPT ( = 5)		LLE ( = 5)		SPE ( = 5)		MAS-M ( = 5)
	Recoveries (%)	RSD (%)	Recoveries (%)	RSD (%)	Recoveries (%)	RSD (%)	Recoveries (%)	RSD (%)
500 ng/mL	129.32	11.14	85.48	55.90	132.95	19.60	103.21	5.17
2 μg/mL	130.42	2.06	67.05	82.21	94.66	9.21	99.38	5.34