Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation

Table 1

Overview of the various lysis buffer and the number of proteins identified using 1D LC-MS/MS shotgun analysis.

Number	Lysis buffer	Buffer component	Heat	Number of protein ID
1	SDS	1% SDS, 0.5 M TEAB	−	NA.
2	SDS	1% SDS, 0.5 M TEAB	+	622
3	RIPA	50 mM Tris, 150 mM NaCl, 1% NP-40, 1% DOC, and 0.1% SDS	−	NA.
4	Urea	9 M urea	−	NA.
5	2D	7 M urea, 2 M thiourea, 4% CHAPS, 20 mM DTT, and 40 mM Tris	−	NA.
6	DOC	5% DOC	−	NA.
7	DOC	5% DOC	+	823
8	TUTS	25 mM TEAB, 8 M urea, 2% triton X-100, and 0.1% SDS	−	NA.

TEAB: triethylammonium bicarbonate; TUTS: TEAB/urea/triton-X/SDS.