Sample Preparation and Extraction in Small Sample Volumes Suitable for Pediatric Clinical Studies: Challenges, Advances, and Experiences of a Bioanalytical HPLC-MS/MS Method Validation Using Enalapril and Enalaprilat
Table 1
Results for recovery, absolute matrix effect, and process efficiency.
Concentration [ng/mL]
Absolute matrix effect ± S.D. [%]
Mean recovery ± S.D. [%]
Mean process efficiency [%]
Enalapril
0.195
−12.6 ± 11.9
77.1 ± 0.7
67.4
3.13
−8.9 ± 2.2
103.5 ± 3.7
94.3
25
−19.8 ± 4.4
102.9 ± 6.3
77.6
200
−17.5 ± 3.5
92.0 ± 6.9
72.5
Enalaprilat
0.175
−1.5 ± 10.8
99.9 ± 7.8
98.4
2.81
0.3 ± 3.7
118.3 ± 4.6
118.7
22.5
10.5 ± 4.9
102.4 ± 4.7
113.2
180
2.2 ± 3.5
92.9 ± 9.2
94.9
Benazepril
25
−7.2 ± 2.8
76.9 ± 2.3
71.3
Data compiled as mean or mean ± standard deviation (S.D.).
