Stability-Indicating HPLC Determination of Gemcitabine in Pharmaceutical Formulations

Table 1

Linearity data of the proposed method.

Conc. (µg/mL)	Mean peak area (gemcitabine) ()	Mean peak area (IS)	Mean area ratio	Conc. found (µg/mL)	% assay
0.5	11556.2	490138.6	0.02358	0.50	99.0
1.0	22196.2	527242.0	0.04210	1.03	102.6
2.0	40119.6	516202.8	0.07772	2.05	102.3
5.0	99206.8	549256.6	0.18062	4.99	99.9
10.0	197702.2	546987.8	0.36144	10.18	101.8
15.0	298096.4	551610.8	0.54041	15.30	102.0
25.0	496084.0	560316.6	0.88536	25.19	100.8
40.0	791722.0	566686.4	1.39711	39.85	99.6
50.0	987580.6	553478.0	1.78432	50.95	101.9
,