A New HPLC-ELSD Method for Simultaneous Determination of N-Acetylglucosamine and N-Acetylgalactosamine in Dairy Foods

Table 3

Intra- and interday precision and accuracy data of GlcNAc and GalNAc determination ( independent replicates).

Compound	FC	Intraday			Interday
		MAC	RSD	AC	MAC	RSD	AC
GlcNAc	25	24.11	1.2	96.4	25.10	1.7	100.4
	75	75.10	0.4	100.1	74.49	1.6	99.3
	120	121.78	0.7	101.5	121.68	1.0	101.4
	150	158.48	0.3	105.7	153.17	1.3	102.1
GalNAc	10	10.31	2.2	103.1	10.37	1.7	103.7
	25	24.55	2.0	98.2	24.91	1.8	99.6
	50	48.54	1.7	97.1	50.13	1.6	100.3
	100	104.07	0.7	104.1	101.95	1.1	101.9

FC = fortified concentration (mg/mL).
MAC = mean absolute concentration (mg/mL).
RSD = relative standard deviation (%).
AC = accuracy of GlcNAc and GalNAc (%).