Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive

Table 1

Effect of the concentration of SBE-β-CD in aqueous phase.

(mM)
4	52.5	55.7	1.27	1.06
8	42.0	44.7	1.30	1.06
12	33.6	36.0	1.30	1.07
16	32.0	34.0	1.31	1.06

Mobile phase: aqueous buffer/methanol/acetonitrile of 21 : 3 : 1 (v/v/v), concentration of SBE-β-CD in aqueous buffer of 4, 8, 12, and 16 mM, 5 mM sodium dihydrogen phosphate, pH of 2.5, flow rate of 1.0 mL/min, detection wavelength of 240 nm, temperature of 25°C, and , and retention factors of ()-CIT and ()-CIT, respectively; , resolution, and , separation factor.