Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive

Table 2

Effect of the composition of mobile phase.

:  :
22 : 3 : 1	39.9	42.7	1.32	1.07
21 : 3 : 1	28.3	30.3	1.30	1.07
20 : 3 : 1	27.6	29.4	1.24	1.06
21 : 3 : 2	15.3	16.2	1.07	1.06
21 : 3 : 0	72.1	73.4	1.40	1.02

Mobile phase: aqueous buffer/methanol/acetonitrile with various ratios, 12 mM SBE-β-CD, 5 mM sodium dihydrogen phosphate, pH of 2.5, flow rate of 1.0 mL/min, detection wavelength of 240 nm, temperature of 25°C, and , and retention factors of ()-CIT and ()-CIT, respectively; , resolution, and α, separation factor.