Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive

Table 3

Effect of the column temperature.

(°C)
20	34.4	37.2	1.33	1.08
25	31.5	33.8	1.30	1.07
30	28.1	30.0	1.20	1.07
35	24.1	25.6	1.12	1.06

Mobile phase: aqueous buffer/methanol/acetonitrile of 21 : 3 : 1 (v/v/v), concentration of SBE-β-CD in aqueous buffer of 4, 8, 12, and 16 mM, 5 mM sodium dihydrogen phosphate, pH of 2.5, flow rate of 1.0 mL/min, detection wavelength of 240 nm, temperature of 25°C, and , and retention factors of ()-CIT and ()-CIT, respectively; , resolution, and , separation factor.