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International Journal of Analytical Chemistry
Volume 2016, Article ID 3216523, 14 pages
http://dx.doi.org/10.1155/2016/3216523
Review Article

Selective Sensing of Tyrosine Phosphorylation in Peptides Using Terbium(III) Complexes

1Department of Applied Chemistry, School of Engineering, Tokyo University of Technology, 1404-1 Katakuramachi, Hachioji, Tokyo 192-0982, Japan
2Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan
3Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8577, Japan
4Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

Received 9 March 2016; Accepted 28 April 2016

Academic Editor: Günther K. Bonn

Copyright © 2016 Jun Sumaoka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

By using binuclear TbIII complexes, phosphorylation of tyrosine in peptides by protein tyrosine kinases (PTKs) and dephosphorylation by protein tyrosine phosphatases (PTPs) can be successfully visualized in a real-time fashion. The activities of various inhibitors on these enzymes are quantitatively evaluated, indicating a strong potential of the method to efficient screening of eminent inhibitors from a number of candidates.

  1. Supplementary Material