The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products

Figure 1

Specificity test of UMPS gene in tobacco and nontobacco plants. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1: negative control (NTC); lane 2: positive control (PTC); lanes 3–18: Chinese jasmine, alfalfa, Altingia, canola, Pittosporum, Daphniphyllum, mondo grass, sapodilla, garden petunia, castor oil, indica rice, coriander, spinach, pomegranate, watermelon, and eggplant; lane M: Trans 2K DNA marker. Ct was expressed as mean Ct SD from 3 independent experiments with three replications.

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