The Development of DNA Based Methods for the Reliable and Efficient Identification of<i> Nicotiana tabacum</i> in Tobacco and Its Derived Products

<div>Sensitivity test of<i> UMPS</i> gene using serial dilutions of genomic DNA from fresh tobaccoleave samples. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis.<i> Lane 1</i>: NTC;<i> lanes 2–9</i>: 120, 60, 30, 15, 7.5, 3.75, 1.88, and 0.94 ng per reaction, respectively;<i> lane M</i>: Trans 2K DNA marker. (c) qPCR method. For standard curve, a serial dilution of DNA samples (120, 60, 30, 15, 7.5, 3.75, 1.88, 0.94, 0.47, 0.24, 0.12, and 0.06 ng) was used. The result was developed after considering 3 independent experiments with three replications.</div>

International Journal of Analytical Chemistry

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Figure 2: The Development of DNA Based Methods for the Reliable and Efficient Identification of<i> Nicotiana tabacum</i> in Tobacco and Its Derived Products 

Figure 2 | The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products 