The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products

Table 1

Descriptions of plant samples used in this study.

Plant materials	Description of the plant materials	Providers
Nontobacco plants; (fresh leaves)	Alfalfa (Medicago sativa); Altingia (Altingia gracilipes); canola (Brassica campestris); castor (Ricinus communis); Chinese jasmine (Jasminum officinale); coriander (Coriandrum sativum); Daphniphyllum (Daphniphyllum teijsmannii); eggplant (Solanum melongena); garden petunia (Petunia × hybrid); Indica rice (Oryza sativa var. indica); mondo grass (Ophiopogon japonicus); Pittosporum (Pittosporum tobira); pomegranate (Punica granatum); sapodilla (Manilkara zapota); spinach (Spinacia oleracea); watermelon (Citrullus lanatus)	Locally collected
Dried tobacco leaves	Burley (3); Oriental SAADI-6; Virginia (2); Oriental (5); reconstituted tobacco foiled (1); Virginia grade A (1); Virginia grade B (1); Oriental BASMAK	Bulgarian customs
Fresh tobacco leaves	Burley; Maryland	Locally collected
Reconstituted tobacco	Factory sample I (tobacco stems, no sulphate cellulose); factory sample II (no tobacco stems, no sulphate cellulose); factory sample III (tobacco stems, with sulphate cellulose); commercial wrapper	Dutch customs
Tobacco stem	Tobacco stems	Bulgarian customs
Cigarettes	Liqun, Double Happiness, Mevius, Marlboro, Wuyeshen	Locally purchased

The numbers in brackets indicate the numbers of tobacco varieties of this species tested in the experiment.