Development of an Ion-Pairing Reagent and HPLC-UV Method for the Detection and Quantification of Six Water-Soluble Vitamins in Animal Feed

Figure 2

HPLC chromatograms of a mixture of six vitamins detected at 270 nm. (1) Nicotinic acid, (2) nicotinamide, (3) pyridoxine, (4) folic acid, (5) riboflavin, and (6) thiamin.