International Journal of Analytical Chemistry

Research Article

Development of an Ion-Pairing Reagent and HPLC-UV Method for the Detection and Quantification of Six Water-Soluble Vitamins in Animal Feed

Table 1

Validation parameters for the developed RP-HPLC/UV method.


Compound	RT	Slope	Intercept		LOD (μg/kg)	LOQ (μg/kg)	Rs	Asymm	Precisions (% RSD)		Recovery (%)
Compound	RT	Slope	Intercept		LOD (μg/kg)	LOQ (μg/kg)	Rs	Asymm	Intraday ()	Interday ()	Recovery (%)

Nicotinic acid	6.4	1.3365	−7.2254 × 10⁻¹	0.9999	31	105	—	1.07	2.6	3.0	94.5
Nicotinamide	11.6	2.3354	−5.4255 × 10⁻¹	0.9999	25	84	19.42	0.97	2.2	2.1	93.5
Pyridoxine	14.2	8.5554 × 10⁻¹	−2.33664	0.9998	197	658	10.20	0.99	2.2	3.0	90.6
Folic acid	18.8	1.22248	1.00014 × 10⁻¹	0.9997	104	347	21.49	0.99	3.0	3.3	82.3
Riboflavin	20.1	2.65487	−5.66541 × 10⁻¹	0.9999	33	110	5.55	0.94	5.6	4.8	95.2
Thiamin	28.3	3.26548 × 10⁻¹	−1.02548 × 10⁻¹	0.9997	28	94	34.85	1.08	4.0	4.2	98.9

Retention time (min).
Coefficients of correlation.
Limit of detection, the lowest analyte concentration that produces a response detectable above the noise level of the system.
Limit of quantification, the lowest level of analyte that can be accurately and precisely measured.
Resolution.
Asymmetry.
Relative standard, expressed as %.
Average of recoveries at two spike levels (high and low).