Development of an Ion-Pairing Reagent and HPLC-UV Method for the Detection and Quantification of Six Water-Soluble Vitamins in Animal Feed

Table 3

Determination of the vitamin contents (mg/kg) in animal feed using the proposed method.

Sample	Nicotinic acid	Nicotinamide	Folic acid	Pyridoxine	Riboflavin	Thiamine
1	5,231	6,380	—	20,449	—	10,648
2	1,868	42,085	102	—	—	9,803
3	20,009	—	—	9,027	4,093	—
4	46,289	—	452	—	29,344	8,014
5	9,227	8,263	—	10,382	30,298	—
6	19,263	—	780	37,030	—	5,226
7	—	49,920	—	2,009	—	5,814
8	—	2,892	358	—	10,023	20,687
9	16,354	34,893	—	5,934	3,015	—
10	8,308	—	552	—	23,589	—
11	5,360	7,099	837	—	24,263	18,893
12	—	10,298	960	9,982	—	4,300
13	6,993	26,407	200	6,057	10,360	—
14	21,960	48,062	—	2,918	5,430	9,366
15	—	—	753	—	—	48,930
16	—	6,337	200	49,530	—	1,380
17	40,826	12,086	—	—	5,669	4,085
18	3,698	—	901	8,006	7,802	30,569
19	—	1,339	5,012	—	9,829	—
20	—	—	303	39,807	4,369	—
21	30,158	2,580	—	5,980	—	10,230
22	4,803	—	807	—	36,820	5,628
23	10,009	—	650	48,301	—	7,083
24	—	15,369	—	2,065		8,292
25	25,801	—	—	—	6,043	—
26	26,990	—	268	—	8,823	1,359
27	9,045	—	—	4,6933	—	4,860
28	4,960	9,963	—	—	—	—
29	—	2,860	—	10,297	6,099	—
30	18,203	5,802	692	4,950	—	20,560