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International Journal of Analytical Chemistry
Volume 2016, Article ID 8480963, 5 pages
http://dx.doi.org/10.1155/2016/8480963
Research Article

Determination of Natamycin in Turkish Yoghurt

Faculty of Pharmacy, Department of Analytical Chemistry, Marmara University, P.O. Box 34668, Haydarpasa, Istanbul, Turkey

Received 8 January 2016; Accepted 6 April 2016

Academic Editor: Josep Esteve-Romero

Copyright © 2016 Dilek Bilgic Alkaya and Ozlem Karalomlu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303 nm. Natamycin was spiked into handmade yoghurt samples and used for validation. The method has been fully validated according to ISO 9233-2, 2007 (IDF 140-2, 2007). It was successfully applied to determination of 28 different Turkish yoghurt products. Findings dealing with the presence of natamycin in cheese samples are presented.