Research Article

Simultaneous Determination of Four Active Ingredients in Sargentodoxa cuneata by HPLC Coupled with Evaporative Light Scattering Detection

Table 2

Recoveries of spiked analytes.

AnalytesOriginal (mg)Spiked (mg)Found (mg)Recovery (%)Mean (%)RSD (%)

3,4-Dihydroxyphenylethyl alcohol glycoside1.2971.875 ± 0.0078100.59 ± 0.93
1.3021.0401.905 ± 0.0049103.71 ± 0.5998.122.38
1.2961.898 ± 0.0191103.45 ± 2.29
1.2992.078 ± 0.003599.84 ± 0.34
1.2921.3002.049 ± 0.019897.63 ± 1.90102.581.69
1.2942.017 ± 0.007194.40 ± 0.68
1.3102.336 ± 0.0057100.35 ± 0.45
1.3201.5602.337 ± 0.0057101.15 ± 0.45102.550.69
1.2972.308 ± 0.0184104.26 ± 1.47

Salidroside1.0391.494 ± 0.0057102.21 ± 0.97
1.0430.8001.492 ± 0.0035100.00 ± 1.41103.620.94
1.0381.500 ± 0.008596.30 ± 0.71
1.0411.651 ± 0.0078103.56 ± 0.88
1.0351.0001.628 ± 0.0113102.67 ± 0.5599.503.00
1.0371.600 ± 0.0057104.63 ± 1.33
1.0491.855 ± 0.0134105.76 ± 1.40
1.0571.2001.823 ± 0.0141101.81 ± 1.47104.642.35
1.0391.852 ± 0.0014106.33 ± 0.15

Chlorogenic acid1.3481.985 ± 0.0212104.93 ± 2.46
1.3541.0801.997 ± 0.0198105.76 ± 2.29103.642.88
1.3471.944 ± 0.0078100.22 ± 0.90
1.3512.179 ± 0.0021101.64 ± 0.20
1.3431.3502.133 ± 0.006497.97 ± 0.59100.682.36
1.3462.183 ± 0.0057102.43 ± 0.52
1.3622.469 ± 0.0099106.44 ± 0.76
1.3731.6202.438 ± 0.0163103.33 ± 1.25104.361.72
1.3482.418 ± 0.0233103.33 ± 1.80

Liriodendrin0.8861.255 ± 0.0071100.40 ± 1.30
0.8900.6801.263 ± 0.0028101.35 ± 0.52101.611.34
0.8851.269 ± 0.0057103.08 ± 1.04
0.8881.363 ± 0.000795.92 ± 0.10
0.8820.8501.368 ± 0.003597.28 ± 0.5296.390.80
0.8841.360 ± 0.004295.97 ± 0.62
0.8951.539 ± 0.0007100.81 ± 0.09
0.9021.0201.531 ± 0.008599.20 ± 1.0499.231.57
0.8861.506 ± 0.005797.70 ± 0.69