Research Article
Simultaneous Determination of Four Active Ingredients in Sargentodoxa cuneata by HPLC Coupled with Evaporative Light Scattering Detection
Table 2
Recoveries of spiked analytes.
| Analytes | Original (mg) | Spiked (mg) | Found (mg) | Recovery (%) | Mean (%) | RSD (%) |
| 3,4-Dihydroxyphenylethyl alcohol glycoside | 1.297 | | 1.875 ± 0.0078 | 100.59 ± 0.93 | | | 1.302 | 1.040 | 1.905 ± 0.0049 | 103.71 ± 0.59 | 98.12 | 2.38 | 1.296 | | 1.898 ± 0.0191 | 103.45 ± 2.29 | | | 1.299 | | 2.078 ± 0.0035 | 99.84 ± 0.34 | | | 1.292 | 1.300 | 2.049 ± 0.0198 | 97.63 ± 1.90 | 102.58 | 1.69 | 1.294 | | 2.017 ± 0.0071 | 94.40 ± 0.68 | | | 1.310 | | 2.336 ± 0.0057 | 100.35 ± 0.45 | | | 1.320 | 1.560 | 2.337 ± 0.0057 | 101.15 ± 0.45 | 102.55 | 0.69 | 1.297 | | 2.308 ± 0.0184 | 104.26 ± 1.47 | | |
| Salidroside | 1.039 | | 1.494 ± 0.0057 | 102.21 ± 0.97 | | | 1.043 | 0.800 | 1.492 ± 0.0035 | 100.00 ± 1.41 | 103.62 | 0.94 | 1.038 | | 1.500 ± 0.0085 | 96.30 ± 0.71 | | | 1.041 | | 1.651 ± 0.0078 | 103.56 ± 0.88 | | | 1.035 | 1.000 | 1.628 ± 0.0113 | 102.67 ± 0.55 | 99.50 | 3.00 | 1.037 | | 1.600 ± 0.0057 | 104.63 ± 1.33 | | | 1.049 | | 1.855 ± 0.0134 | 105.76 ± 1.40 | | | 1.057 | 1.200 | 1.823 ± 0.0141 | 101.81 ± 1.47 | 104.64 | 2.35 | 1.039 | | 1.852 ± 0.0014 | 106.33 ± 0.15 | | |
| Chlorogenic acid | 1.348 | | 1.985 ± 0.0212 | 104.93 ± 2.46 | | | 1.354 | 1.080 | 1.997 ± 0.0198 | 105.76 ± 2.29 | 103.64 | 2.88 | 1.347 | | 1.944 ± 0.0078 | 100.22 ± 0.90 | | | 1.351 | | 2.179 ± 0.0021 | 101.64 ± 0.20 | | | 1.343 | 1.350 | 2.133 ± 0.0064 | 97.97 ± 0.59 | 100.68 | 2.36 | 1.346 | | 2.183 ± 0.0057 | 102.43 ± 0.52 | | | 1.362 | | 2.469 ± 0.0099 | 106.44 ± 0.76 | | | 1.373 | 1.620 | 2.438 ± 0.0163 | 103.33 ± 1.25 | 104.36 | 1.72 | 1.348 | | 2.418 ± 0.0233 | 103.33 ± 1.80 | | |
| Liriodendrin | 0.886 | | 1.255 ± 0.0071 | 100.40 ± 1.30 | | | 0.890 | 0.680 | 1.263 ± 0.0028 | 101.35 ± 0.52 | 101.61 | 1.34 | 0.885 | | 1.269 ± 0.0057 | 103.08 ± 1.04 | | | 0.888 | | 1.363 ± 0.0007 | 95.92 ± 0.10 | | | 0.882 | 0.850 | 1.368 ± 0.0035 | 97.28 ± 0.52 | 96.39 | 0.80 | 0.884 | | 1.360 ± 0.0042 | 95.97 ± 0.62 | | | 0.895 | | 1.539 ± 0.0007 | 100.81 ± 0.09 | | | 0.902 | 1.020 | 1.531 ± 0.0085 | 99.20 ± 1.04 | 99.23 | 1.57 | 0.886 | | 1.506 ± 0.0057 | 97.70 ± 0.69 | | |
|
|