International Journal of Analytical Chemistry / 2016 / Article / Fig 2

Research Article

Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil

Figure 2

Positive ESI-MS/MS product ion spectra of (a) glucosamine and (b) galactosamine, produced through direct infusion of 100 μM reference solutions of each analyte with a collision energy of 25 V. Suggested fragmentation of glucosamine (c) presenting the formation of the two m/z transitions investigated in the method (180 72 and 180 84). Fragmentation of galactosamine is believed to follow the same route but with less formation of the 84 fragment due to the conformation of the C4 hydroxy group.
(a)
(b)
(c)

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