Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil

Table 1

In and output values of the full factorial design used to investigate the linear relationship of peak area for the 72 and 84 Th fragments in relation to known concentrations of glucosamine (GlcN) and galactosamine (GalN). Experiments were analyzed in random order.

	Conc.^1 (M)		Peak area (cps)		Calculated conc.^2 (M)
Experiment	GlcN	GalN	180 > 72	180 > 84	GlcN	GalN
1	1	5	3.6E5	3.3E4	1	5
2	1	10	5.8E5	4.1E4	1	10
3	1	15	9.9E5	6.1E4	1	16
4	8	5	5.3E5	1.3E5	9	5
5	8	10	8.0E5	1.4E5	9	10
6	8	15	1.2E6	1.7E5	9	16
7	15	5	6.9E5	2.2E5	16	5
8	15	10	9.7E5	2.3E5	16	10
9	15	15	1.4E6	2.8E5	16	15
10	1	5	3.3E5	2.9E4	1	5
11	1	10	6.0E5	4.1E4	1	11
12	1	15	1.0E6	6.1E4	1	16
13	8	5	5.9E5	1.5E5	9	5
14	8	10	9.0E5	1.6E5	9	11
15	8	15	1.2E6	1.7E5	8	15
16	15	5	7.9E5	2.6E5	16	5
17	15	10	9.8E5	2.4E5	17	10
18	15	15	1.3E6	2.5E5	16	15

Concentrations of GlcN and GalN prepared for each experiment
GlcN and GalN concentrations calculated using (3) and (4) with slope coefficients () derived from coefficients of slope from calibration curves determined from standard solutions analyzed within the same acquisition batch.