Research Article
Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil
Table 3
LC-MS/MS method performance of retention, linearity, sensitivity, precision, limit of detection, and limit of quantification for glucosamine, galactosamine, and muramic acid.
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Pearson correlation coefficient () determined for seven points, ranging from LOQ to 40 M (). Regression slope expressed in area units (counts)/M (). Precision expressed as relative standard deviation (RSD) for repeated injections at 1 M (L) and 10 M (H) (). Limit of detection reported as the injected concentration giving a peak height corresponding to three times the baseline noise level. Limit of quantification reported as the injected concentration giving a peak height corresponding to ten times the baseline noise level. Precision at low level was investigated at a concentration of 2 M for the 180 > 84 transition of galactosamine, due to its relatively high LOD. |