International Journal of Analytical Chemistry / 2016 / Article / Tab 3

Research Article

Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil

Table 3

LC-MS/MS method performance of retention, linearity, sensitivity, precision, limit of detection, and limit of quantification for glucosamine, galactosamine, and muramic acid.

Analyte transition (min)LinearitySensitivityPrec. L/H (%)LOD (M)LOQ (M)

Glucosamine180 > 724.41 ± 0.040.99973.8E4 ± 0.33E41.3/1.00.0250.07
Glucosamine180 > 844.41 ± 0.040.99972.1E4 ± 0.17E40.8/0.70.050.2
Galactosamine180 > 724.52 ± 0.030.99998.1E4 ± 0.78E40.3/0.70.010.03
Galactosamine180 > 844.52 ± 0.030.99973.8E3 ± 0.38E31.8/1.50.51.2
Muramic acid252 > 1262.01 ± 0.010.99945.1E4 ± 0.20E41.0/0.60.010.03

Pearson correlation coefficient () determined for seven points, ranging from LOQ to 40 M ().
Regression slope expressed in area units (counts)/M ().
Precision expressed as relative standard deviation (RSD) for repeated injections at 1 M (L) and 10 M (H) ().
Limit of detection reported as the injected concentration giving a peak height corresponding to three times the baseline noise level.
Limit of quantification reported as the injected concentration giving a peak height corresponding to ten times the baseline noise level.
Precision at low level was investigated at a concentration of 2 M for the 180 > 84 transition of galactosamine, due to its relatively high LOD.

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