Simultaneous Determination of Bergapten, Imperatorin, Notopterol, and Isoimperatorin in Rat Plasma by High Performance Liquid Chromatography with Fluorescence Detection and Its Application to Pharmacokinetic and Excretion Study after Oral Administration of Notopterygium incisum Extract

Table 4

Stability of bergapten, imperatorin, notopterol, and isoimperatorin in rat plasma ().

Compounds	Concentration	Freeze-thaw cycles		Autosampler for 24 h		At −80°C for 1 month
	(ng mL^−1)	Mean (%)	RSD (%)	Mean (%)	RSD (%)	Mean (%)	RSD (%)
Bergapten	4	106	11.1	100	10.2	80.5	4.65
	12	91.1	6.09	101	12.9	91.1	9.57
	60	83.6	7.69	115	6.93	104	6.40
	600	85.1	5.83	88.9	6.99	112	4.05
Imperatorin	40	93.7	14.7	105	8.13	113	6.86
	120	93.9	4.74	115	4.21	85.7	7.24
	600	106	4.02	89.6	3.92	98.3	8.85
	6000	100	4.65	114	1.85	104	4.78
Notopterol	4	102	14.7	104	12.1	92.3	12.5
	12	93.2	10.1	109	13.6	88.6	8.38
	60	97.1	9.76	97.3	9.39	105	9.86
	600	94.9	2.39	102	5.61	103	5.18
Isoimperatorin	2	93.9	7.40	96.9	12.2	82.2	15.0
	6	106	9.71	119	10.0	112	9.12
	30	85.7	7.80	98.1	9.41	111	7.96
	300	85.8	5.80	88.8	7.34	103	5.72