Isocratic Resolution of Fluoroquinolone-Based Antibiotics on the Phenylethyl-Bonded Phase under Nonaqueous Elution: A Consideration of the Separation Mechanism

Table 3

Theoretical interaction simulation results between 6 selected antibiotics and 3 stationary phase modifiers.

	Phenyl			Phenylethyl			Phenylhexyl
	( kJ/mol)			( kJ/mol)			( kJ/mol)
Difloxacin
Enrofloxacin
Ofloxacin
Pipemidic acid
Cinoxacin
Nalidixic acid

analyte and the modifier of stationary phase are first minimized in energy before placing the modifier around the analyte to calculate the energy released upon interaction based on the semiempirical method. These collected energy values are then categorized into the defined intervals. number of the released energy values that fall in the interval.