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International Journal of Analytical Chemistry
Volume 2018, Article ID 1605950, 9 pages
Research Article

LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction

1Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur 522002, Andhra Pradesh, India
2College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna Nagar 522510, Guntur, Andhra Pradesh, India
3Clinical Pharmacology and Bio Sciences Division, RA Chem Pharma, Hyderabad, India
4College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA

Correspondence should be addressed to Pappula Nagaraju; moc.liamg@ujaraganaluppap

Received 11 March 2018; Revised 11 June 2018; Accepted 11 July 2018; Published 28 October 2018

Academic Editor: Barbara Bojko

Copyright © 2018 Pappula Nagaraju et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An accurate, highly sensitive, and precise method for quantitative analysis of tramadol (TMD) and gabapentin (GBP) by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using venlafaxine and pregabalin as internal standards (ISTDs), respectively. An aliquot of 200 μL of plasma was mixed with internal standard dilution and extraction was performed by using solid phase extraction (SPE) technique. Peak resolution was achieved on Phenomenex PFP column (50×4.6 mm, 2.6 μm). The total analytical run time was 3.8 min. Both analytes were monitored using multiple reaction monitoring (MRM) scan and the mass spectrometer was operated in positive polarity mode. The method was validated for specificity, sensitivity, precision, accuracy, and other analytical parameters. The results found were satisfactory over the linear calibration range of 1-500 ng/mL and 10-6000 ng/mL for TMD and GBP, respectively. The developed method can be ready to use by scientific community for quantification of analytes in plasma samples from various clinical studies of different dose strengths.