Development and Validation of a Simple High Performance Liquid Chromatography/UV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine

Figure 1

HPLC chromatograms of (a) standards of uric acid (75 μg/mL), creatinine (75 μg/mL), internal standard, tyrosine (125 μg/mL), and hypoxanthine (75 μg/mL). 1, uric acid (5.2 min); 2, creatinine (6.0 min); 3, tyrosine the internal standard (7.1 min); 4, hypoxanthine (8.2 min). (b) HPLC chromatogram of human urine.

(a)

(b)