Development and Validation of a Simple High Performance Liquid Chromatography/UV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine

Table 1

Regression equation obtained for interday and intraday variation of uric acid, creatinine, and hypoxanthine.

Experiment	Analyte	Regression equation
Intraday variation	Uric acid		0.998
	Creatinine		0.999
	Hypoxanthine		0.999
Interday variation	Uric acid		0.995
	Creatinine		0.998
	Hypoxanthine		0.997

, peak area ratio (peak area of the analyte to that of the internal standard); , concentration of the analyte; , correlation coefficient.