Development and Validation of a Simple High Performance Liquid Chromatography/UV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine
Table 2
Accuracy, repeatability, and precision for uric acid, creatinine, and hypoxanthine.
Accuracy (recovery%)
Repeatability CV%
Precision (CV%)
Intraday ()
Interday ()
Uric acid (μg/mL)
12.5
98.17 ± 0.57
0.35
0.74
4.62
25
100.12 ± 0.16
0.50
1.20
3.84
75
99.49 ± 0.13
0.15
0.53
1.64
Creatinine (μg/mL)
12.5
97.52 ± 0.38
0.95
1.88
4.40
25
101.44 ± 0.21
0.56
0.59
4.58
75
99.84 ± 0.42
0.81
0.73
1.41
Hypoxanthine (μg/mL)
12.5
100.32 ± 1.75
0.35
0.31
3.53
25
100.43 ± 1.02
0.63
0.37
3.13
75
100.40 ± 0.15
0.19
1.64
1.40
CV%, coefficient of variation; , number of replications of analyte samples.
