A Green and Efficient Method for the Preconcentration and Determination of Gallic Acid, Bergenin, Quercitrin, and Embelin from Ardisia japonica Using Nononic Surfactant Genapol X-080 as the Extraction Solvent

Figure 6

The typical HPLC chromatograms of (a) the mixed standard solutions: (1) gallic acid, 50 μg mL⁻¹; (2) bergenin, 500 μg mL⁻¹; (3) quercitrin, 50 μg mL⁻¹; (4) embelin, 250 μg mL⁻¹; (b) sample extracted by ultrasonic assisted micellar extraction and then preconcentrated by cloud point extraction: (1) gallic acid, 5.58 μg mL⁻¹; (2) bergenin, 216.65 μg mL⁻¹; (3) quercitrin, 12.72 μg mL⁻¹; (4) embelin, 48.45 μg mL⁻¹; (c) sample extracted by ultrasonic assisted micellar extraction. (1) gallic acid, 1.09 μg mL⁻¹; (2) bergenin, 39.93 μg mL⁻¹; (3) quercitrin, 2.59 μg mL⁻¹; (4) embelin, 10.46 μg mL⁻¹.

(a)

(b)

(c)