Research Article
Biomarker Profiling for Pyridoxine Dependent Epilepsy in Dried Blood Spots by HILIC-ESI-MS
Table 1
Method performance specifications for AASA/P6C and pipecolic acid.
| Analyte | Performance specifications | Calibration range (g/mL)Linearity | LOQ (g/mL) | LOD (g/mL) | QC Levels (g/mL) | Accuracy () | Precision () | Percent Recovery () | Inter | Intra | Inter | Intra |
| AASA/P6C | 0.05-10 (r2=0.999) | 0.05 | 0.01 | LLOQ (0.05) | 92.06 | 99.71 | 3.43 | 3.12 | - | LQC (0.5) | 101.98 | 100.15 | 5.16 | 5.31 | 80.22 | MQC (1) | 100.78 | 96.78 | 3.60 | 4.16 | 80.88 | HQC (8) | 101.32 | 94.12 | 7.87 | 5.12 | 85.12 |
| Pipecolic acid | 0.05-9 (r2=0.997) | 0.05 | 0.01 | LLOQ (0.05) | 100.10 | 99.22 | 2.25 | 3.78 | - | LQC (0.5) | 98.10 | 100.87 | 1.66 | 5.00 | 90.33 | MQC (1) | 100.32 | 100.21 | 3.38 | 2.84 | 93.23 | HQC (8) | 98.17 | 98.74 | 2.34 | 2.31 | 92.16 |
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LOQ: limit of quantification, LOD: limit of detection, LLOQ: lower limit of quantification, LQC: low quality control, MQC: medium quality control, HQC: high quality control, AASA: alpha-amino adipic semialdehyde, and P6C: piperidine-6-carboxylic acid.
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