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International Journal of Analytical Chemistry
Volume 2018, Article ID 4271385, 12 pages
https://doi.org/10.1155/2018/4271385
Research Article

Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis

1Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, Xi’an, Shaanxi 710069, China
2Shaanxi Institute for Food and Drug Control, Xi’an, Shaanxi 710069, China
3Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China

Correspondence should be addressed to Wei Cao; nc.ude.uwn@iewoac

Received 2 September 2017; Revised 25 October 2017; Accepted 20 November 2017; Published 1 January 2018

Academic Editor: Troy D. Wood

Copyright © 2018 Haiyan Lei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study reports an analytical method for the determination of nitroimidazole and quinolones in honey using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified QuEChERS methodology was used to extract the analytes and determine veterinary drugs in honey by LC-MS/MS. The linear regression was excellent at the concentration levels of 1–100 ng/mL in the solution standard curve and the matrix standard curve. The recovery rates of nitroimidazole and quinolones were 4.4% to 59.1% and 9.8% to 46.2% with relative standard deviations (RSDs) below 5.2% and the recovery rates of nitroimidazole and quinolones by the matrix standard curve ranged from 82.0% to 117.8% and 79% to 115.9% with relative standard deviations (RSDs) lower than 6.3% in acacia and jujube honey. The acacia and jujube honeys have stronger matrix inhibition effect to nitroimidazole and quinolones residue; the matrix inhibition effect of jujube honey is stronger than acacia honey. The matrix standard curve can calibrate matrix effect effectively. In this study, the detection method of antibiotics in honey can be applied to the actual sample. The results demonstrated that the modified QuEChERS method combined with LC-MS/MS is a rapid, high, sensitive method for the analysis of nitroimidazoles and quinolones residues in honey.