Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on<i> Spirulina platensis</i>

<div>Representative photographs of 2D thin layer chromatograms for a methanolic extract of<i> Spirulina platensis</i>, developed with the indicated mobile phases and visualized under visible light (VL) and under ultraviolet light (UVL<sub>366</sub>) (central column) and the correspondent 2D thin layer biochromatograms, obtained after DPPH treatment at t<sub>0</sub> and <svg height="10.9562pt" id="M151" style="vertical-align:-3.18152pt" version="1.1" viewbox="-0.0498162 -7.77468 9.51004 10.9562" width="9.51004pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M298 36L289 62C276 55 253 45 228 45C202 45 169 60 169 141V397H276C289 405 292 426 282 437H169V574L155 576L90 509V437H45L17 408L21 397H90V107C90 28 125 -12 188 -12C198 -12 213 -8 230 1L298 36Z" id="g121-114"></path></g><g transform="matrix(.0091,0,0,-0.0091,3.965,3.132)"><path d="M502 500C497 551 492 613 491 650H43V617C122 612 130 606 130 521V129C130 45 122 38 41 33V0H319V33C227 38 217 45 217 129V305H319C411 305 413 295 427 225H461V421H427C414 357 411 348 319 348H217V580C217 609 219 612 250 612H330C401 612 423 605 439 578C452 557 458 539 467 496L502 500Z" id="g58-68"></path></g></svg> (right column).</div>

International Journal of Analytical Chemistry

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Figure 2

Figure 2: Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on<i> Spirulina platensis</i> 

Figure 2 | Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis 