Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis

Table 1

Relative volumetric proportion for the mobile phases used to perform the 1D-TLCs and 2D-TLCs.

Mobile phase	Solvent 1(δ1): Solvent 2 (δ2): modifier	Relative volumetric proportion
MP-1	AcO (20.3): n-Hx (14.9): non-used	3:7:0
MP-2	AcO (20.3): n-Hx (14.9): non-used	1:1:0
MP-3	AcO (20.3): EA (18.6): non-used	1:1:0
MP-4a	IOH (23.5): EA (18.6): non-used	0.16:1:0
MP-4b	IOH (23.5): EA (18.6): non-used	1:1:0
MP-4c	IOH (23.5): EA (18.6): non-used	1:0.16:0
MP-5a	IOH (23.5): EA (18.6): NH4O	0.16:1:0.25
MP-5b	IOH (23.5): EA (18.6): NH4O	1:1:0.25
MP-5c	IOH (23.5): EA (18.6): NH4O	1:0.16:0.25

Solubility parameter (; expressed in (J/cm³)^1/2) [38]. = 4.75.