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International Journal of Analytical Chemistry
Volume 2018, Article ID 4650764, 8 pages
Research Article

Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography

1Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kasetsart University, 10900, Bangkok, Thailand
2Center for Advanced Studies for Agriculture and Food, KU Institute for Advanced Studies, Kasetsart University (CASAF, NRU-KU), 10900, Bangkok, Thailand
3Faculty of Veterinary Medicine, Kamphaengsaen Campus, 73140, Nakhon Pathom, Thailand
4Kasetsart University Research and Development Institute, 10900, Bangkok, Thailand

Correspondence should be addressed to Natthasit Tansakul; moc.oohay@ttisahttan

Received 16 March 2018; Accepted 10 May 2018; Published 24 June 2018

Academic Editor: Mohamed Abdel-Rehim

Copyright © 2018 Sasiprapa Choochuay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A reliable and rapid method has been developed for the determination of aflatoxin B1 (AFB1) in four kinds of feedstuffs comprising broken rice, peanuts, corn, and fishmeal. A sample preparation was carried out based on the QuEChERS method with the exclusion of the clean-up step. In this study, AFB1 was extracted using acetonitrile/methanol (40/60 v/v), followed by partitioning with sodium chloride and magnesium sulfate. High-performance liquid chromatography with precolumn derivatization and fluorescence detection was performed. The coefficients of determination were greater than 0.9800. Throughout the developed method, the recovery of all feedstuffs achieved a range of 82.50-109.85% with relative standard deviation lower than 11% for all analytes at a concentration of 20-100 ng/g. The limit of detection (LOD) ranged from 0.2 to 1.2 ng/g and limit of quantitation (LOQ) ranged from 0.3 to 1.5 ng/g. The validated method was successfully applied to a total of 120 samples. The occurrence of AFB1 contamination was found at the following concentrations: in broken rice (0.44-2.33ng/g), peanut (3.97-106.26ng/g), corn (0.88-50.29 ng/g), and fishmeal (1.06-10.35 ng/g). These results indicate that the proposed method may be useful for regularly monitoring AFB1 contamination in feedstuffs.